beta Actin antibody (AA 350-375) (Biotin)

Catalog No. ABIN964578

Supplier Product No.: 600-406-886

This anti-beta Actin antibody is a Rabbit Polyclonal antibody detecting beta Actin in WB, ELISA and IHC. Suitable for Human, Mouse, Rat, Monkey, Pig, Cow, Sheep, Hamster, Guinea Pig and Goat.

Quick Overview for beta Actin antibody (AA 350-375) (Biotin) (ABIN964578)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Monkey, Pig, Cow, Sheep, Hamster, Guinea Pig, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-beta Actin Antibody (Biotin)

Binding Specificity: AA 350-375

Supplier Product No.: 600-406-886

Supplier: Rockland

Purpose: Beta Actin Antibody Biotin Conjugated

Cross-Reactivity (Details): This affinity-purified antibody is directed against human beta Actin protein.

Characteristics: Synonyms: rabbit anti-beta Actin Antibody biotin conjugation, biotin conjugated rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Optional[Immunogen]: beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 350-375 of Human beta Actin.

Isotype: IgG

Labeling Ratio: 10-20

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: This affinity-purified antibody has been tested for use in ELISA and is suitable for immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly.  Beta actin present in neuromuscular junctions also stains.  Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation.  Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB

Background: Background: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility.  Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Biotin Conjugate Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.

Gene ID: 60

NCBI Accession: NP_001092

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction